Review



paper zenodo repository  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc paper zenodo repository
    Paper Zenodo Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+zenodo+repository/pLA+CMV+N-Flag+RALB+V23+R47+(Plasmid+%2350983)/pm34348148-168-107-139
    Average 90 stars, based on 1 article reviews
    paper zenodo repository - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Saline:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Electrophoresis:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Membrane:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Staining:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    cDNA Synthesis:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    RNA Sequencing:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Recombinant:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Plasmid Preparation:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Software:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Imaging:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Spectrophotometry:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Polymerase Chain Reaction:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Confocal Microscopy:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046

    Microscopy:

    Article Title: Severe deficiency of the voltage-gated sodium channel Na V 1.2 elevates neuronal excitability in adult mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SCN2A (NaV1.2) Alomone Labs Cat#ASC-002; RRID: AB_2040005 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174S; RRID: RRID: AB_10622025 b-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat#3700S; RRID: RRID: AB_2242334 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences P/N: 926-68071; RRID: AB_10956166 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences P/N: 926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rat IgG Secondary Antibody LI-COR Biosciences P/N: 926-68076; RRID: AB_10956590 Bacterial and virus strains pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene 55634-AAVrg PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH This paper; Penn Vector Core N/A PHP.eB-Ef1a-DO-mCherry-WPRE-pA This paper; Bio-Detail Corporation N/A Chemicals, peptides, and recombinant proteins NEUROBIOTIN Tracer Vector Laboratories SP-1120 Streptavidin, Alexa Fluor 488 Conjugate Thermo Fisher Scientific S32354 4% paraformaldehyde Thermo Fisher Scientific AAJ19943K2 TRIzol Reagent Thermo Fisher Scientific 15596018 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Protease Inhibitor Mini Tablets Thermo Fisher Scientific A32953 Pierce Rapid Gold BCA Protein Assay Kit Thermo Fisher Scientific A53226 NuPAGE LDS Sample Buffer (4 3 ) Thermo Fisher Scientific NP0007 NuPAGE Sample Reducing Agent (10 3 ) Thermo Fisher Scientific NP0009 PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Fisher Scientific 26619 PowerUp SYBR Green Master Mix Thermo Fisher Scientific A25777 SYBR Safe DNA Gel Stain Invitrogen by Thermo Fisher Scientific S33102 Tween 20 Fisher Scientific AAJ20605AP Formaldehyde solution Sigma-Aldrich F8775 Glutaraldehyde solution Sigma-Aldrich 354400 4-(Trifluoromethyl)-L-phenylglycine Sigma-Aldrich 16599 Choline chloride Sigma-Aldrich C7527 Bovine Serum Albumin Sigma-Aldrich A9418 1 kb DNA Ladder Sigma-Aldrich D0428-1VL Agarose Sigma-Aldrich A9539 Tetrodotoxin citrate Abcam ab120055 CGP 55845 hydrochloride Abcam ab120337 D-AP5 Abcam ab120003 CNQX disodium salt Abcam ab120044 ZD7288 Abcam ab120102 Pimaric acid MP Biomedicals 219895105 (2-Fluorophenyl) glycine VWR 101795-980 Odyssey Blocking Buffer (PBS) LI-COR Biosciences 927-40000 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences 927-65001 (Continued on next page) e1 Cell Reports 36, 109495, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER 10 3 Tris Buffered Saline Bio-Rad 1706435 10 3 Tris/Glycine Buffer Bio-Rad 1610771 10 3 Tris/Glycine/SDS Electrophoresis Buffer Bio-Rad 1610772 Immobilon -FL PVDF membrane Millipore IPFL00010 50 3 pH 8,3 tris-acetate EDTA buffer Millipore 1.06174 a-Dendrotoxin Alomone Labs D-350 Tetrodotoxin citrate Alomone Labs T-550 Picrotoxin Alomone Labs P-325 Optimal Cutting Temperature compound Tissue Tek 4583 NucleoSpin DNA RapidLyse Macherey-Nagel 740100.250 Liquid Proteinase K Macherey-Nagel 740396 Critical commercial assays b-Gal Staining Set Sigma-Aldrich 11828673001 Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase Thermo Fisher Scientific K1672 Deposited data Raw and processed RNA sequencing data This paper GEO: GSE179818 Custom scripts This paper Zenodo Repository https://zenodo. org/record/5098353 Experimental models: Organisms/strains Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl The National Laboratory Animal Center Rodent Model Resource Center Scn2aWT/gt Oligonucleotides See Table S1 N/A Recombinant DNA pAAV-EF1a-mCherry-IRES-Flpo Fenno et al., 2014 Addgene Plasmid # 55634 pUCmini-iCAP-PHP.eB Chan et al., 2017 Addgene plasmid # 103005 pAAV-Ef1a-DO-mCherry-WPRE-pA Saunders et al., 2012 Addgene plasmid # 37119 Software and algorithms ImageJ (Fiji) Eliceiri/LOCI group https://fiji.sc/ Image Studio Lite 5.2 LI-COR Biosciences https://www.licor.com/bio/image-studio-lite/d5 pClamp 11.1 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system OriginPro version 2020 OriginLab Corporation https://www.originlab.com/ GraphPad Prism version 8.0.0 for Windows GraphPad Software https://www.graphpad.com/ Adobe Illustrator 2020 Adobe https://www.adobe.com/ Other Odyssey CLx Imaging System LI-COR Biosciences https://www.licor.com/bio/odyssey-clx/ NanoDrop OneC Microvolume UV-Vis Spectrophotometer Thermo Fisher Scientific N/A C1000 Touch PCR thermal cycler Bio-Rad N/A MultiPhoton confocal microscopy Nikon A1R-MP LSM 900 scanning confocal microscope Zeiss N/A Cryostat Leica Biosystems CM1950 Vibrating blade microtome Leica Biosystems VT1200 S Micropipette Puller Sutter Instrument P-1000 Borosilicate glass with filament Sutter Instrument BF150-110-10 Axon MultiClamp 700B Microelectrode Amplifier Molecular Devices N/A Axon Digidata 1550B Plus HumSilencer Molecular Devices Digidata 1550B4 (Continued on next page) Cell Reports 36, 109495, August 3, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER BX-51WI microscope Olympus N/A IR-2000 Infrared Monochrome Video Camera Dage-MTI N/A Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) RWD Life Science Inc. 68046



    Similar Products

    paper zenodo repository  (Addgene inc)
    90
    Addgene inc paper zenodo repository
    Paper Zenodo Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+zenodo+repository/pLA+CMV+N-Flag+RALB+V23+R47+(Plasmid+%2350983)/pm34348148-168-107-139
    Average 90 stars, based on 1 article reviews
    paper zenodo repository - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results